The effect of acoustic wave propagating in the direction of temperature gradient on a stability of stationary plane-parallel flow of binary fluid, generated by horizontal temperature and concentration gradients in a horizontal layer is investigated for two types of thermal boundary conditions: perfectly conductive and adiabatic boundaries. The study is performed analytically for the longwave perturbations and numerically for finite-wavelength perturbations. Stability maps for fixed Prandtl number value Pr = 0.01 and different values of Schmidt number and acoustic Reynolds number are obtained. The most dangerous instability modes and critical perturbation structure are discussed. Comparison with the case where acoustic wave is absent is performed. It is shown, that longwave perturbations are always stabilized by acoustic wave. Numerical investigation demonstrates, that acoustic wave also stabilizes the flow with respect to finite-wavelength perturbations when negative concentrational Grashof number is higher in modulus than certain value depending on acoustic wave intensity. (C) 2018 Elsevier Ltd. All rights reserved.