This paper presents the improved method of analysis and evaluation of the silver layers' adhesion printed by inkjet printing technology. The generally known method for adhesion measurement of layers and coatings according to the standards ASTM D3359 and ISO 2409 has been improved by development of the automatic evaluation software of achieved results from cross cut tests. The developed evaluation tool offers the possibilities of calculation the detached area, rotation of the image, setting the threshold and the automatic classification according to the mentioned standards. The inaccuracy caused by samples' assessment by humans is mainly eliminated by using of this tool. The developed evaluation tool has been applied to experimental works oriented to analysis of the adhesion of two commercially available silver based nano-inks on two polymeric based substrates (PI and PET). For this purpose, different sintering conditions have been applied to analysed samples. The achieved results show the differences between the investigated nano-inks' adhesion on two types of polymeric substrates. The adhesion of silver layers can be optimized by controlling the sintering conditions. By developing the software tool the simple and inaccurate method of adhesion analysis has been upgraded to the appropriate technique to the silver layers' adhesion testing. The improved method for adhesion measurements can serve as a tool for fast and relatively simple technique for technologist.