Aims (i) To determine the aflatoxin B-1 (AFB(1)) adsorption and desorption dynamics in the presence of Lactobacillus rhamnosus RC007 under simulated transit of AFB(1) at each gastrointestinal tract (GIT-saliva, stomach and intestine) stage consecutively and then, separately, (ii) to study the ability of L. rhamnosus RC007 to biotransform AFB(1) as a strategy that complements the adsorption process. Methods and Results The AFB(1) adsorption and desorption assay simulating the GIT passage of AFB(1) (93 center dot 89 ng g(-1)) in the presence of L. rhamnosus RC007 (10(8) CFU per ml) was conducted. Moreover, lactic acid production was determined. Results demonstrated that predominant environmental conditions in salivary solution induced a low AFB(1) adsorption, while the transit through the gastric solution and intestinal solution allowed high percentages of adsorption and did not generate significant AFB(1) desorption. Conclusions The AFB(1) adsorption and desorption dynamics in the presence of L. rhamnosus RC007 was favoured by gastric and intestinal environment. Significance and Impact of the Study The knowledge of the adsorption dynamics of AFB(1) with a micro-organism of interest will allow predicting its behaviour at each stage of the GIT.