We report a calorimetric study of the binding in aqueous solution of some guests (dichloromethane, furan, tetrahydrofuran, 1,4- dioxane and tetrahydropyran) with the cage of the supramolecular iron-organic host ((Fe4L6)-L-II)(4-), where L is formed by 2-formylpyridine and 4,4'-diaminobiphenyl-2,2'-disulfonic acid. This supramolecular iron-organic host has a tetrahedral structure with a cage capable to complex specific guests. The study was carried out by using an isothermal titration calorimeter, allowing the determination of the binding constants between some of the guests with ((Fe4L6)-L-II)(4-). Depending on the molecular nature of the guests their incorporation can be fast or very slow. In this latter case, the rate of energy released during the process was used to determine the kinetic constant for the assemblage between the guests with ((Fe4L6)-L-II)(4-). For the cases of fast encapsulation, the correspondent values for Delta H-298(0), Delta HG(298)(0) and Delta S-298(0) were determined and the thermodynamic driven force was elucidated. (C) 2018 Elsevier Ltd.