Recently, the design of biomimetic enzymes based on ultrathin two-dimension (2D) metal-organic framework (MOF) nanosheets have attracted great attention due to their large surface area and abundant accessible active sites. In this work, ultrathin 2D MOF nanosheets and multi-walled carbon nanotubes (MWCNTs) are simply mixed to prepare the composite (2D MOF/MWCNT) films though a Langmuir-Schafer method. The 2D MOF/MWCNT films are easily transferred on electrodes as a novel electrochemical platform for the detection of hydrogen peroxide (H2O2), with a detection limit of 0.70 mu M (S/N = 3), a wide linear range from 0.001 to 8.159 mM, a high sensitivity of 157 mu A cm(-2)mM(-1), a short response time of < 3 s, excellent selectivity and high stability. Compared to pure ultrathin 2D MOF nanosheets, the introduction of MWCNTs can improve the sensitivity of composite films because of the increase of electrical conductivity. The facile, sensitive, stable and selective H2O2 sensors are proven to be appropriate for the determination of H2O2 concentration in real samples such as serum and beer.