Electrochemical oxidation of haematoxylin (HX), a bioactive bis-catechol molecule was studied at poly(alanine) modified carbon paste electrode (CPE) by using cyclic voltammetric technique followed by its electrochemical characterisation. The poly(alanine) modified CPE exhibited a high sensitivity towards the oxidation of HX. The effect of scan rate was found to be adsorption-controlled. The concentration study in the range of 20-160 mu M was calibrated and the limit of detection (3S/M) and limit of quantification (10S/M) were calculated to be 0.102 mu M and 0.34 mu M respectively. Because of the high sensitivity of the fabricated poly(alanine) modified CPE it can be used as a promising electrochemical sensor for the determination of HX.