This study attempts to prompt the formation of microorganism films on flexible textile-based anodes and enhances the performance of living microorganisms by introducing magnetic properties to the anodes. A magnetic and electrically conductive anode for a microbial fuel cell is designed and fabricated by encapsulating uniformly dispersed SrFe12O19 nanoparticles into the poly(vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibers and forming a three-dimensional (3D) polypyrrole (PPy) network on the surface of flexible composite nanofiber based fabric. A dual-chamber MFC equipped with the magnetized anode shows a maximum power density of 3317 mW m(-2), which is significantly larger than that of the non-magnetized anode (2471 mW m(-2)). This study demonstrates that the hard-magnetic anode providing an inherent magnetic field can greatly promote bio-electrochemical reaction rates of E. colt and decrease the anode charge transfer resistance in a MFC system.