The fruit of Spondias tuberosa (umbu) is widely consumed in the northeast region of Brazil. In this work, the potential of umbu seeds as source of bioactive compounds was evaluated. Different extraction techniques were compared in terms of yield, free-fatty acids composition, total phenolic content (TPC) and antioxidant activity (AA). The extraction techniques used were supercritical fluid extraction (SFE) with CO2, performed at 40 degrees C and pressures from 15 to 30 MPa, and Soxhlet and ultrasound-assisted extraction (UAE) with different solvents. The highest yields were obtained by UAE with ethanol/water mixtures and by Soxhlet with ethanol. The higher values for TPC and AA were observed for the extracts obtained with polar solvents. Furthermore, a biorefinery approach considering integrated processes (SFE + UAE) in order to recover polyphenols from the SFE residues has also been investigated and the results showed that combining processes may lead to a most effective valorization of agro-industrial residues.