Thiols possess a crucial role in the formation of the complex three-dimensional protein structure and are helpful in the inhibition of cellular apoptosis. Enhanced concentration of some thiols can be an indicator of diseases such as cancer, angiogenesis, AIDS, and cardiovascular disease. The chemical details of the living cell and correlation between thiol quantity and associated properties have been of interest to the medical community. It was observed that fluorescein arsenical helix binder -ethanedithiol fluorescence is several times higher than its binding with peptide. Some ethanedithiol derivatives are used to prevent biofilm formation by Proteus Mirabilis and Staphylococcus Epidermidis on medical devices, and hence reducing infections. Similarly, the use of dodecanethiol (DDT) is common in the fabrication of hydrophobic self-assembled monolayers (SAMs), sensors, derivatization applications, and environment monitoring. In this report, two different thiols (ethanedithiol and dodecanethiol) were detected using CoPc (cobalt phthalocyanine) -black-P type hybrid sensors. Electrochemical tests of thiols-based buffers using specially designed electrodes such as Si-Ag-Black P-CoPc and AgIC-Ag-Black-P-CoPc were performed. Many of these electrodes exhibited a systematic response when thiol concentration was varied from similar to 1.0 to 5.0 mu L/mL. Vapor phase real time sensing of the thiol also exhibited very good performance. The Randles-Sevcik equation, which linearly relates the peak current and the square root of potential scan rate as observed in cyclic voltammetry tests, was used in performance evaluations. Electrochemical impedance spectroscopy results suggest that CoPc coating helps to enhance electron transfer capability of black-P. (C) 2019 The Electrochemical Society.