Commercially available azo dyes bearing amino groups were grafted to zwitterionic copolymers composed of cyclic anhydride functionality. The zwitterionic copolymers were prepared for the first time by polymerizing sulfobetaine (SB) monomer with maleic anhydride (MA) under conventional free radical polymerization as well as reversible addition-fragmentation chain transfer (RAFT) polymerization. Poly(SB-co-MA) self-assembled in deionized water. Azobenzene grafted zwitterionic poly((SB-co-MA)-g-Azo) exhibited multiresponsive behavior. As confirmed by UV-vis spectroscopy, trans -> cis isomerization of the azo group was responsible for the photo- and thermal responsive behavior. The photoisomerization was reversible, and no photoaging was detected during the repeated exposure to UV and visible light. The water-soluble nature of photoresponsive azo dye grafted copolymers makes it suitable for applications in biological systems.