Chromatographic separation is a promising alternative for the purification of xylo-oligosaccharides (XOSs), a functional food additive in the form of an oligomeric saccharide. DOWEX MONOSPHERE (TM) 99/310 resin ionized with K+, which has better selectivity compared with Ca2+ and Na+, was used as the stationary phase. Breakthrough experiments showed that xylo-oligosaccharides and the two major industrial impurities, xylose and arabinose, all exhibit linear isotherms. Transport-dispersive (TD) model parameters were determined by pulse experiments carried out at various flowrates. In order to acquire model parameters for XOS7, of which the standard sample was not available, elution profiles of XOS containing XOS2 to XO57 were compared with those of individual XOS2 to XOS6. The result shows that XOS7 has adsorption behaviors like XOS6. The difference in Henry's constant, decreasing from 0.335 for XOS2 to 0.0155 for XOS6 and XOS7, may have significant effects on computational design of the chromatographic separation process. Experimental results for both single column and sequential simulated moving bed (SSMB) processes were used to validate the acquired TD model parameters of individual components.