Nano-aerosols of size 100 nm and below are manifested everywhere in various urban micro-environments. In outdoor, the vehicular emission during traffic jam can release nano-aerosols with concentration above 200 million/m(3) (200/cm(3)). Superimposed on vehicular emission are smog particles, around 10-50 nm, generated from the photochemical reaction of hydrocarbon and NOx in the presence of sunlight. These nano-aerosols, by virtue of their small sizes, can be inhaled readily into our bodies leading possibly to various chronic diseases. Airborne viruses from influenza to epidemic viruses, which can lead to acute sickness and even death, are also in the same size range of 100 nm. Despite microfibers are being commonly used today in filters, there has been limited studies on filtration of real aerosols using microfiber filters, let alone nanofibrous filters, as most studies used simulated aerosols (e.g. sodium chloride) or test dust. Also, in standard sodium chloride test, only monodispersed aerosol size is allowed to challenge the filter. On the other hand, in reality aerosols of all sizes challenge simultaneously the filter. In this study, for the first time we have used nanofiber filter in a portable test filter set-up to filter polydispersed aerosols from the micro-environment near busy traffic area where aerosols comprise of both vehicular and atmospheric pollutants with size range between 10 and 400 nm. Real-time measurements are carried out with the portable test filter. The test results are compared to the theoretical correlation from Payet with diffusion correction at small Peclet number (Pe). We have found good comparison between test results with theoretical correlations using equivalent aerodynamic diameter for the aerosols with face velocity from 1 to 11 cm/s. A new dimensionless parameter, specific filter resistance (also established independently by Buckingham-pi approach), is defined for the first time. It measures the flow drag on fibers to the amount of fibers present in the filter, the lower is the specific filter resistance the better is the filter. For our test condition, it is approximately 1.0-1.2. We have also improved the filtration efficiency of the filter by increasing the fiber basis weight by stacking up two layers of nanofibers. We have demonstrated that the efficiency increases while both the quality factor and specific filter resistance remain constant. We have also investigated the single fiber efficiency due to diffusion and found that at low velocity and low Peclet number (Pe < 10), the test results agree well with the theoretical prediction. On the other hand, at higher face velocity (> 0.07 m/s) and large Peclet number (> 10), there is small deviation from the theory, which is probably due to the smaller aerosols collecting by the larger aerosols upstream of the filter with an airstream consisting of polydispersed aerosol distribution challenging the filter. This has not been realized previously as tests conducted were mostly using monodispersed aerosol size distribution for which aerosol-aerosol interaction was absent.