솔잎 추출물이 삽입된 마그네슘-층상규산염 샌드위치 나노입자의 합성과 피부 상재균에 대한 항균 특성에 관한 연구

김성열; 최유성; Seong Yeol Kim; Yoo-Sung Choi

Applied Chemistry for Engineering, Vol.30, No.2, 254-259, April, 2019

DOI10.14478/ace.2019.1011 Export Citation

솔잎 추출물이 삽입된 마그네슘-층상규산염 샌드위치 나노입자의 합성과 피부 상재균에 대한 항균 특성에 관한 연구

Study on Synthesis of Pine Leaf Extract Intercalated Mg-Phyllosilicate Sandwich Nanoparticles and Antimicrobial Activity against Cutaneous Microorganisms

김성열, 최유성^†

(주)럭키산업 연구개발팀

Seong Yeol Kim, and Yoo-Sung Choi^†

Lucky Research and Development Center (LRDC), Lucky Industry Co., Ltd., Gwangsan-gu, Gwangju 62230, Korea

E-mail:

초록

본 연구에서는 마그네슘-층상규산염(MgP) 층간에 솔잎 추출물(PLE)이 삽입되어 겹겹이 쌓여있는 샌드위치 구조의 나노입자(PLE/MgP)를 one-pot으로 합성하였다. XRD 분석을 통해 팔면체와 사면체 구조로 MgP가 성공적으로 합성되었으며, PLE가 층간에 삽입되어 MgP 간의 층간거리(d-spacing)가 벌어진 것을 확인하였다. HR-TEM을 통해 모폴로지 변화를 관찰하였으며, 열중량분석법을 통해 PLE가 MgP 대비 10%까지 함유된 것을 확인하였다. 최소저해농도(MIC) 및 최소 사멸농도(MBC) 분석을 통해 피부 상재균에 대한 항균 활성을 검토한 결과 MgP 또는 PLE보다 PLE/MgP의 항균 활성이 더욱 향상된 것을 확인하였다. 이러한 결과는 본 연구에서 개발한 PLE/MgP 유/무기 복합 소재가 화장품 소재, 의료용 소재 등의 다양한 분야에 적용 가능함을 확인하였다.

In this study, we synthesized the pine leaf extract intercalated layered Mg-phyllosilicate nanoparticles (PLE/MgP) via one-pot synthesis. MgP was successfully synthesized with the octahedral and tetrahedral structure by XRD analysis and a gap of interlayer distance (d-spacing) between MgP sheets by the intercalation of PLE was confirmed. As a result of the investigation of antimicrobial activity against cutaneous microorganisms by the minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) analyses, the antimicrobial activity of PLE/MgP was more improved than that of MgP or PLE. The prepared sandwich-structured PLE/MgP organic/inorganic hybrid materials will be useful in the field of numerous applications containing cosmetic and biomedical materials.

Keywords:Pine leaf extract;Mg-phyllosilicate;Intercalation;Antimicrobial;Cutaneous microorganism

[References]

Grice EA, Segre JA, Nat. Rev. Microbiol., 9, 244 (2011)
Nakatsuji T, Chen TH, Narala S, Chun KA, Two AM, et al., Sci. Transl. Med., 9, eaah4680 (2017).
Kobayashi T, Glatz M, Horiuchi K, Kawasaki H, Akiyama H, Kaplan DH, Kong HH, Amagai M, Nagao K, Immunity, 42, 756 (2015)
Dessinioti C, Katsambas A, Clin. Dermatol., .2, 163 (2017)
Aditya A, Chattopadhyay S, Jha D, Gautam HK, Maiti S, Ganguli M, ACS Appl. Mater. Interfaces, 10, 15401 (2018)
Comune M, Rai A, Chereddy KK, Pinto S, Aday S, et al., J. Control. Release, 262, 58 (2017)
Aljuffali IA, Huang CH, Fang JY, Curr. Drug Metab., 16, 255 (2015)
Dhanalakshmi V, Nimal TR, Sabitha M, Biswas R, Jayakumar R, J. Biomed. Mater. Res. B, 104, 797 (2016)
Yim MH, Hong TG, Lee JH, Food Sci. Biotechnol., 15, 582 (2006)
Si W, Gong J, Tsao R, Kalab M, Yang R, Yin Y, J. Chromatogr. A, 1125, 204 (2006)
Wilson B, Abraham G, Manju VS, Mathew M, Vimala B, Sundaresan S, Nambisan B, J. Ethnopharmacol., 99, 147 (2005)
Yan D, Jin C, Xiao XH, Dong XP, J. Biochem. Biophys. Methods, 70, 845 (2008)
Koo H, Gomes BPFA, Rosalen PL, Ambrosano GMB, Park YK, Cury JA, Arch. Oral Biol., 45, 141 (2000)
Han Y, Li P, Xu Y, Li H, Song Z, Nie Z, Chen Z, Yao S, Small, 11(7), 877 (2015)
Koukos PK, Papadopoulou KI, Patiaka DT, Papagiannopoulos AD, J. Agric. Food Chem., 48, 1266 (2000)
Lim YS, Bae MJ, Lee SH, J. Korean Soc. Food Sci. Nutr., 31, 333 (2002)
Zeng WC, Zhang ZZ, Gao H, Jia LR, He Q, J. Food. Sci., 77, C824 (2012)
Wu YP, Liang X, Liu XY, Zhong K, Gao B, Huang YN, Gao H, J. Funct. Foods, 14, 605 (2015)
Yen GC, Duh PD, Huang DW, Hsu CL, Fu TYC, Food Chem. Toxicol., 46, 175 (2008)
Choy JH, Jung JS, Oh JM, Park M, Jeong J, Kang YK, Han OJ, Biomaterials, 25, 3059 (2004)
Kim JS, Park I, Jeong ES, Jin K, Seong WM, Yoon G, Kim H, Kim B, Nam KT, Kang K, Adv. Mater., 29(21), 160689 (2017)
Gu P, Zhang S, Li X, Wang X, Wen T, Jehan R, Alsaedi A, Hayat T, Wang X, Environ. Pollut., 240, 493 (2018)
Han HK, Lee YC, Lee MY, Patil AJ, Shin HJ, ACS Appl. Mater. Interfaces, 3, 2564 (2011)
Liu LM, Jiang LP, Liu F, Lu GY, Abdel-Halim ES, Zhu JJ, Anal. Methods, 5, 3565 (2013)
Schmidt MP, Martinez CE, Langmuir, 32(31), 7719 (2016)
Yang JH, Lee JH, Ryu HJ, Elzatahry AA, Alothman ZA, Choy JH, Appl. Clay Sci., 130, 20 (2016)
Zhang Y, Long M, Huang P, Yang H, Chang S, Hu Y, Tang A, Mao L, Sci. Rep., 6, 33335 (2016)
Haraguchi K, Polym. J., 43, 223 (2011)
He H, Ma Y, Zhu J, Yuan P, Qing Y, Appl. Clay Sci., 48, 67 (2010)
Lee Y, Choi Y, Choi M, Yang H, Liu K, Shin H, Appl. Clay Sci., 83-84, 474 (2013)
Chandrasekaran G, Han HK, Kim GJ, Shin HJ, Appl. Clay Sci., 53, 729 (2011)
Holley RA, Patel D, Food Microbiol., 22, 273 (2005)
Zeng WC, He Q, Sun Q, Zhong K, Gao H, Int. J. Food Microbiol., 153, 78 (2012)

[Referenced By]

[1] Grice EA, Segre JA, Nat. Rev. Microbiol., 9, 244 (2011)

[2] Nakatsuji T, Chen TH, Narala S, Chun KA, Two AM, et al., Sci. Transl. Med., 9, eaah4680 (2017).

[3] Kobayashi T, Glatz M, Horiuchi K, Kawasaki H, Akiyama H, Kaplan DH, Kong HH, Amagai M, Nagao K, Immunity, 42, 756 (2015)

[4] Dessinioti C, Katsambas A, Clin. Dermatol., .2, 163 (2017)

[5] Aditya A, Chattopadhyay S, Jha D, Gautam HK, Maiti S, Ganguli M, ACS Appl. Mater. Interfaces, 10, 15401 (2018)

[6] Comune M, Rai A, Chereddy KK, Pinto S, Aday S, et al., J. Control. Release, 262, 58 (2017)

[7] Aljuffali IA, Huang CH, Fang JY, Curr. Drug Metab., 16, 255 (2015)

[8] Dhanalakshmi V, Nimal TR, Sabitha M, Biswas R, Jayakumar R, J. Biomed. Mater. Res. B, 104, 797 (2016)

[9] Yim MH, Hong TG, Lee JH, Food Sci. Biotechnol., 15, 582 (2006)

[10] Si W, Gong J, Tsao R, Kalab M, Yang R, Yin Y, J. Chromatogr. A, 1125, 204 (2006)

[11] Wilson B, Abraham G, Manju VS, Mathew M, Vimala B, Sundaresan S, Nambisan B, J. Ethnopharmacol., 99, 147 (2005)

[12] Yan D, Jin C, Xiao XH, Dong XP, J. Biochem. Biophys. Methods, 70, 845 (2008)

[13] Koo H, Gomes BPFA, Rosalen PL, Ambrosano GMB, Park YK, Cury JA, Arch. Oral Biol., 45, 141 (2000)

[14] Han Y, Li P, Xu Y, Li H, Song Z, Nie Z, Chen Z, Yao S, Small, 11(7), 877 (2015)

[15] Koukos PK, Papadopoulou KI, Patiaka DT, Papagiannopoulos AD, J. Agric. Food Chem., 48, 1266 (2000)

[16] Lim YS, Bae MJ, Lee SH, J. Korean Soc. Food Sci. Nutr., 31, 333 (2002)

[17] Zeng WC, Zhang ZZ, Gao H, Jia LR, He Q, J. Food. Sci., 77, C824 (2012)

[18] Wu YP, Liang X, Liu XY, Zhong K, Gao B, Huang YN, Gao H, J. Funct. Foods, 14, 605 (2015)

[19] Yen GC, Duh PD, Huang DW, Hsu CL, Fu TYC, Food Chem. Toxicol., 46, 175 (2008)

[20] Choy JH, Jung JS, Oh JM, Park M, Jeong J, Kang YK, Han OJ, Biomaterials, 25, 3059 (2004)

[21] Kim JS, Park I, Jeong ES, Jin K, Seong WM, Yoon G, Kim H, Kim B, Nam KT, Kang K, Adv. Mater., 29(21), 160689 (2017)

[22] Gu P, Zhang S, Li X, Wang X, Wen T, Jehan R, Alsaedi A, Hayat T, Wang X, Environ. Pollut., 240, 493 (2018)

[23] Han HK, Lee YC, Lee MY, Patil AJ, Shin HJ, ACS Appl. Mater. Interfaces, 3, 2564 (2011)

[24] Liu LM, Jiang LP, Liu F, Lu GY, Abdel-Halim ES, Zhu JJ, Anal. Methods, 5, 3565 (2013)

[25] Schmidt MP, Martinez CE, Langmuir, 32(31), 7719 (2016)

[26] Yang JH, Lee JH, Ryu HJ, Elzatahry AA, Alothman ZA, Choy JH, Appl. Clay Sci., 130, 20 (2016)

[27] Zhang Y, Long M, Huang P, Yang H, Chang S, Hu Y, Tang A, Mao L, Sci. Rep., 6, 33335 (2016)

[28] Haraguchi K, Polym. J., 43, 223 (2011)

[29] He H, Ma Y, Zhu J, Yuan P, Qing Y, Appl. Clay Sci., 48, 67 (2010)

[30] Lee Y, Choi Y, Choi M, Yang H, Liu K, Shin H, Appl. Clay Sci., 83-84, 474 (2013)

[31] Chandrasekaran G, Han HK, Kim GJ, Shin HJ, Appl. Clay Sci., 53, 729 (2011)

[32] Holley RA, Patel D, Food Microbiol., 22, 273 (2005)

[33] Zeng WC, He Q, Sun Q, Zhong K, Gao H, Int. J. Food Microbiol., 153, 78 (2012)