Human hair keratin-based hydrogels as dynamic matrices for facilitating wound healing

So Yeon Kim; Bong Joo Park; Yunki Lee; Na Jeong Park; Kyung Min Park; Yu-Shik Hwang; Ki Dong Park

Journal of Industrial and Engineering Chemistry, Vol.73, 142-151, May, 2019

DOI10.1016/j.jiec.2019.01.017 Export Citation

Human hair keratin-based hydrogels as dynamic matrices for facilitating wound healing

So Yeon Kim^{1, 2}, Bong Joo Park³, Yunki Lee⁴, Na Jeong Park⁴, Kyung Min Park⁴, Yu-Shik Hwang^{1, †}, and Ki Dong Park^{4, †}

¹Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
²Department of Dental Hygiene, College of Health Science, Cheongju University, Cheongju 360-764, Republic of Korea
³Department of Electrical and Biological Physics and Institute of Biomaterials, Kwangwoon University, Seoul 01897, Republic of Korea
⁴Department of Molecular Science and Technology, Ajou University, Suwon 16499, Republic of Korea

E-mail:,

Recently, human hair-derived keratin protein has been recognized as biomaterial with high potential due to its excellent bioactivity and biocompatibility. Here, we designed human hair-derived keratin-based in situ cross-linkable hydrogels that can serve as a dynamic matrix for the enhanced wound healing. We demonstrated that our developed the keratin-based hydrogels accelerated re-epithelization and wound healing process in a full-thickness animal. Also, we investigated the molecular mechanism underlying the enhanced wound healing. In conclusion, our study proposes that human hair-derived keratin-based hydrogels with excellent bioactivity have great potential for use as a wound healing material, along with its other biomedical applications.

Keywords:Polymeric hydrogels;Hair keratin;Wound healing;Keratinocyte;Migration;Epithelial-Mesenchyme Transition

[References]

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Fonder MA, Lazarus GS, Cowan DA, Aronson-Cook B, Kohli AR, Mamelak AJ, J. Am. Acad. Dermatol., 58, 185 (2008)
Lee Y, Choi KH, Park KM, Lee JM, Park BJ, Park KD, ACS Appl. Mater. Interfaces, 9, 16890 (2017)
Tran NQ, Joung YK, Lih E, Park KD, Biomacromolecules, 12(8), 2872 (2011)
GhavamiNejad A, Park CH, Kim CS, Biomacromolecules, 17(3), 1213 (2016)
Giano MC, Ibrahim Z, Medina SH, Sarhane KA, Christensen JM, Yamada Y, Brandacher G, Schneider JP, Nat. Commun., 5, 4095 (2014)
Mi L, Xue H, Li YT, Jiang SY, Adv. Funct. Mater., 21(21), 4028 (2011)
Park KM, Gerecht S, Nat. Commun., 5, 4075 (2014)
Park S, Park KM, Biomaterials, 182, 234 (2018)
Mutsaers SE, Bishop JE, McGrouther G, Laurent GJ, Int. J. Biochem. Cell Biol., 29, 5 (1997)
Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, Patel SB, Khalid L, Isseroff RR, Tomic-Canic M, Adv. Wound Care, 3, 445 (2014)
Doillon CJ, Whyne CF, Brandwein S, Silver FH, J. Biomed. Mater. Res., 20, 1219 (1986)
Lin K, Zhang D, Macedo MH, Cui W, Sarmento B, Shen G, Adv. Funct. Mater., 1804943 (2018).
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Ellingsworth LR, DeLustro F, Brennan JE, Sawamura S, McPherson J, J. Immunol., 136, 877 (1986)
Siegle RJ, McCoy JP, Schade W, Swanson NA, Arch. Dermatol., 120, 183 (1984)
Diamond AM, Gorham SD, Etherington DJ, Robertson JG, Light ND, Matrix, 11, 321 (1991)
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Pechter PM, Gil J, Valdes J, Tomic-Canic M, Pastar I, Stojadinovic O, Kirsner RS, Davis SC, Wound Repair Regen., 20, 236 (2012)
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Schrooyen PM, Dijkstra PJ, Oberthur RC, Bantjes A, Feijen J, J. Agric. Food Chem., 48, 4326 (2000)
Lee Y, Park KM, Bae JW, Park KD, Colloids Surf. B: Biointerfaces, 102, 585 (2013)
Park KM, Ko KS, Joung YK, Shin H, Park KD, J. Mater. Chem., 21, 13180 (2011)
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O’Toole EA, Clin. Exp. Dermatol., 26, 525 (2001)
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Wheelock MJ, Jensen PJ, J. Cell Biol., 117, 415 (1992)
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[Referenced By]

[1] Fullenkamp DE, Rivera JG, Gong YK, Lau KH, He L, Varshney R, Messersmith PB, Biomaterials, 33, 3783 (2012)

[2] Fonder MA, Lazarus GS, Cowan DA, Aronson-Cook B, Kohli AR, Mamelak AJ, J. Am. Acad. Dermatol., 58, 185 (2008)

[3] Lee Y, Choi KH, Park KM, Lee JM, Park BJ, Park KD, ACS Appl. Mater. Interfaces, 9, 16890 (2017)

[4] Tran NQ, Joung YK, Lih E, Park KD, Biomacromolecules, 12(8), 2872 (2011)

[5] GhavamiNejad A, Park CH, Kim CS, Biomacromolecules, 17(3), 1213 (2016)

[6] Giano MC, Ibrahim Z, Medina SH, Sarhane KA, Christensen JM, Yamada Y, Brandacher G, Schneider JP, Nat. Commun., 5, 4095 (2014)

[7] Mi L, Xue H, Li YT, Jiang SY, Adv. Funct. Mater., 21(21), 4028 (2011)

[8] Park KM, Gerecht S, Nat. Commun., 5, 4075 (2014)

[9] Park S, Park KM, Biomaterials, 182, 234 (2018)

[10] Mutsaers SE, Bishop JE, McGrouther G, Laurent GJ, Int. J. Biochem. Cell Biol., 29, 5 (1997)

[11] Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, Patel SB, Khalid L, Isseroff RR, Tomic-Canic M, Adv. Wound Care, 3, 445 (2014)

[12] Doillon CJ, Whyne CF, Brandwein S, Silver FH, J. Biomed. Mater. Res., 20, 1219 (1986)

[13] Lin K, Zhang D, Macedo MH, Cui W, Sarmento B, Shen G, Adv. Funct. Mater., 1804943 (2018).

[14] Mian M, Beghe F, Mian E, Int. J. Tissue React., 14, 1 (1992)

[15] Ellingsworth LR, DeLustro F, Brennan JE, Sawamura S, McPherson J, J. Immunol., 136, 877 (1986)

[16] Siegle RJ, McCoy JP, Schade W, Swanson NA, Arch. Dermatol., 120, 183 (1984)

[17] Diamond AM, Gorham SD, Etherington DJ, Robertson JG, Light ND, Matrix, 11, 321 (1991)

[18] Lee H, Noh K, Lee SC, Kwon IK, Han DW, Lee IS, Hwang YS, Tissue Eng. Regen. Med., 11, 255 (2014)

[19] Pechter PM, Gil J, Valdes J, Tomic-Canic M, Pastar I, Stojadinovic O, Kirsner RS, Davis SC, Wound Repair Regen., 20, 236 (2012)

[20] Than P, Smith RA, Cassidy S, Kelly R, Marsh C, Maderal A, Kirsner RS, J. Dermatol. Treat., 24, 290 (2013)

[21] Staron P, Banach M, Kowalski Z, Staron A, Proc. ECOpole, 8 (2014)

[22] Lee H, Hwang YS, Lee HS, Choi S, Kim SY, Moon JH, Kim JH, Kim KC, Han DW, Park HJ, Bae H, Macromol. Res., 23(3), 300 (2015)

[23] Schrooyen PM, Dijkstra PJ, Oberthur RC, Bantjes A, Feijen J, J. Agric. Food Chem., 48, 4326 (2000)

[24] Lee Y, Park KM, Bae JW, Park KD, Colloids Surf. B: Biointerfaces, 102, 585 (2013)

[25] Park KM, Ko KS, Joung YK, Shin H, Park KD, J. Mater. Chem., 21, 13180 (2011)

[26] Luo JD, Wang YY, Fu WL, Wu J, Chen AF, Circulation, 110, 2484 (2004)

[27] Wang X, Ge J, Tredget EE, Wu Y, Nat. Protoc., 8, 302 (2013)

[28] Asai J, Takenaka H, Kusano KF, Ii M, Luedemann C, Curry C, Eaton E, et al., Circulation, 113, 2413 (2006)

[29] Lee Y, Bae JW, Lee JW, Suh W, Park KD, J. Mater. Chem.. B, 2, 7712 (2014)

[30] Santoro MM, Gaudino G, Exp. Cell Res., 304, 274 (2005)

[31] O’Toole EA, Clin. Exp. Dermatol., 26, 525 (2001)

[32] Odland G, Ross R, J. Cell Biol., 39, 135 (1968)

[33] Wheelock MJ, Jensen PJ, J. Cell Biol., 117, 415 (1992)

[34] Mendez MG, Kojima S, Goldman RD, Faseb J., 24, 1838 (2010)

[35] Rasanen K, Vaheri A, J. Dermatol. Sci., 58, 97 (2010)

[36] Cano A, Perez-Moreno MA, Rodrigo I, Locascio A, Blanco MJ, del Barrio MG, Portillo F, Nieto MA, Nat. Cell Biol., 2, 76 (2000)

[37] Yan C, Grimm WA, Garner WL, Qin L, Travis T, Tan N, Han YP, Am. J. Pathol., 176, 2247 (2010)

[38] Kalluri R, Weinberg RA, J. Clin. Invest., 119, 1420 (2009)

[39] Huang X, Wu J, Spong S, Sheppard D, J. Cell Sci., 111, 2189 (1998)

[40] Kim JP, Zhang K, Chen JD, Wynn KC, Kramer RH, Woodley DT, J. Cell. Physiol., 151, 443 (1992)

[41] Quinn JV, Tissue adhesives in clinical medicine, PMPH-USA, 2005.

[42] Roy S, Khanna S, Sen CK, Free Radic. Biol. Med., 44, 180 (2008)