The mutation of Scenedesmus obliquus grown in municipal wastewater by laser combined with ultraviolet

Xu Zhou; Wenbiao Jin; Song-fang Han; Xiaoye Li; Shu-Hong Gao; Chuan Chen; Guo-jun Xie; Renjie Tu; Qing Wang; Qilin Wang

Korean Journal of Chemical Engineering, Vol.36, No.6, 880-885, June, 2019

DOI10.1007/s11814-019-0273-3 Export Citation

The mutation of Scenedesmus obliquus grown in municipal wastewater by laser combined with ultraviolet

Xu Zhou, Wenbiao Jin^†, Song-fang Han, Xiaoye Li, Shu-Hong Gao¹, Chuan Chen², Guo-jun Xie², Renjie Tu, Qing Wang, and Qilin Wang³

Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology (Shenzhen), 518055 Shenzhen, China
¹Department of Microbiology and Plant Biology, University of Oklahoma, Norman, Oklahoma 73019, USA
²State Key Laboratory of Urban Water Resource and Environment (SKLUWRE), Harbin Institute of Technology, 150001, Harbin, China
³Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia

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Mutagenetic breeding is an efficient technique for the enhancement of lipid productivity from microalgae. In this study, oil-rich microalga Scenedesmus obliquus were treated by Laser-UV composite mutagenesis. Among the 35 mutant strains, X5 was primely screened. Afterwards, a twice UV mutagenizing was operated on X5, and the optimal mutant strain X5-H13 was obtained. The growth rate, dry weight, lipid yield and lipid content of X5-H13 were 0.698× 107 cells/mL·d, 0.99 g/L, 0.49 g/L and 48.8% while cultivated in municipal wastewater, respectively, which were increased by 45%, 58%, 109% and 32% than the original strain. The results of the subculture of repeated mutant showed that the biomass and lipid content of strain X5-H13 were up to 0.99 g/L and 48.8%. The growth of each generation was stable. Furthermore, the random amplified polymorphic DNA analysis indicated that the mutant strain X5-H13 was different from the starting strain, with their genetic similarity coefficient value of 0.815.

Keywords:Microalgae;Scenedesmus obliquus;Biodiesel;Laser-UV Mutagenesis;Biomass

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[17] Zhuang H, Chen B, Chen R, Wang M, Shi Q, Acta Laser Biology Sinica, 10, 135 (2001)

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[20] Han SF, Jin WB, Chen YG, Tu RJ, Abomohra A, Appl. Biochem. Biotechnol., 180(6), 1043 (2016)

[21] Ryu BG, Kim EJ, Kim HS, Kim J, Choi YE, Yang JW, Biotechnol. Bioproc. E., 19, 201 (2014)

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