This paper reports on novel nanosized acid resistant material base on the modification of eggshell powder and titanium dioxide (TiO2-EB) for enamel remineralization. The TiO2-EB was prepared by ball milling eggshell powder and titanium dioxide. Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscope (SEM), Transmission Electron Microscopy (TEM), and ImageJ were used to characterise TiO2-EB. A computation model using Material Studio Software was used to explain the mechanism of TiO2-EB interaction. In addition, the acid resistant of TiO2-EB was evaluated by comparison using three commercial toothpaste. The mean pressure value (kPa/s) was measured using a pressure sensor. The FTIR, XRD analysis confirmed the surface modification of TiO2-EB. The SEM image revealed that pure TiO2 particles are spread on the surface of eggshell powder. The TEM image revealed spherical particles in TiO2-EB. The ImageJ showed the average particle size of TiO2-EB to be 13 nm. In addition, the commercial toothpastes doped with TiO2-EB showed an improved acid resistant. The salient features of this study indicate that TiO2-EB will effectively remineralized enamel lesions while offering better protective covering to the enamel. (C) 2019 The Society of Powder Technology Japan. Published by Elsevier B.V. and The Society of Powder Technology Japan. All rights reserved.