Journal of the American Chemical Society, Vol.117, No.9, 2395-2400, 1995
Environmentally Compatible Synthesis of Catechol from D-Glucose
A biocatalytic alternative to currently employed industrial synthesis of catechol has been developed. Klebsiella pneumoniae genes encoding 3-dehydroshikimate dehydratase (aroZ) and protocatechuic acid decarboxylase (aroY) were introduced into an Escherichia coil construct that synthesizes elevated levels of 3-dehydroshikimic acid. One of the resulting biocatalysts, E. coli AB2834/pKD136/pKD9.069A, synthesizes 18.5 +/- 2.0 mM catechol from 56 mM D-glucose on 1 L scale.
Keywords:PLASMID-BASED BIOCATALYSIS;UTILIZATION GENE-CLUSTER;NEUROSPORA-CRASSA;KLEBSIELLA-PNEUMONIAE;MOLECULAR CHARACTERIZATION;ASPERGILLUS-NIDULANS;RECOMBINANT PLASMIDS;COMMON PATHWAY;CLONING;IDENTIFICATION