Pyruvic acid is an important chemical in the carboxylate platform. Obstacles for its implementation are the need for high energy in chemical synthesis and additives in fermentation leading to increased production costs. Here, pyruvic acid generation from direct conversion of lactic acid in fermentation broth by electrolysis method is presented. It was found that lactic acid could be converted to pyruvic acid in the electrolysis cell under alkaline conditions. Using 12.53g/L lactic acid fermentation broth as anolyte, 7.01g/L pyruvic acid could be produced and productivity to lactic acid was 57.66% at initial pH11.74 and 5.0V applied a voltage in the electrolysis cell. Meanwhile, 0.472mol hydrogen was produced at the cathode. The electric energy efficiency was 76.18%. Lactic acid fermentation is relatively cheap and can be performed on many kinds of wastes and biomasses. The results suggest that pyruvic acid production from direct electrolysis of lactic acid fermentation broth can be economically feasible.