ObjectiveTo quantitatively hydroxylate 8S- and 10S-positions on polyunsaturated fatty acids by recombinant Escherichia coli cells expressing mouse arachidonate 8S-lipoxygenase (8S-LOX).ResultsHydroxylated products gained from the conversion of arachidonic acid (20:4(5Z,8Z,11Z,14Z), AA), eicosapentanoic acid (20:5(5Z,8Z,11Z,14Z,17Z), EPA), and (22:6(4Z,7Z,10Z,13Z,16Z,19Z), DHA) by recombinant E. coli cells containing 8S-LOX from mouse were identified as 8S-hydroxy-5,9,11,14(Z,E,Z,Z)-eicosatetranoic acid (8S-HETE), 8S-hydroxy-5,9,11,14,17(Z,E,Z,Z,Z)-eicosapentanoic acid (8S-HEPE), and 10S-hydroxy-4,8,12,14,16,19(Z,E,Z,Z,Z,Z)-docosahexaenoic acid (10S-HDoHE), respectively. Under the optimal hydroxylation conditions of pH 7.5, 30 degrees C, 5% (v/v) ethanol, 15g cells l(-1), and 5mM substrate, AA, EPA, and DHA were hydroxylated into 4.37mM 8S-HETE, 3.77mM 8S-HEPE, and 3.13mM 10S-HDoHE for 60, 90, and 60min, with 87, 75, and 63% molar conversions, respectively.ConclusionTo the best of our knowledge, this is the first quantitatively biotechnological production of 8S-HETE, 8S-HEPE, and 10S-HDoHE.