This study investigated the oxidizing ability of .OH for macro-crude oil in soil using oil-absorbing Fe catalyzing H2O2. Two types of oil-contaminated soil were tested: (i) macro long-chain crude oil-contaminated soil (LO-soil) was dominated by long-chain alkanes, and (ii) macro common crude oil-contaminated soil (CO-soil) was dominated by both short-and long-chain alkanes. LO-soil contained 38% (C-30 - C-27: 1490 mg/kg) macro-crude oil, and CO-soil contained 38% (C-30, C-29, C-15 and C-14: 1473 mg/kg) macro-crude oil. The results showed that the greatest removal of macro-crude oil (LO-soil: 938 mg/kg, CO-soil: 821 mg/kg) was observed by H2O2/oil-absorbing Fe, an increase of twofold compared with by H2O2/non oil-absorbing Fe. In addition, the removal of macro-crude oil by per unit iron species in H2O2/oil-absorbing Fe was as high as 0.25-0.35 mg oil/mg Fe, twice that (0.13-0.17 mg oil/mg Fe) achieved by H2O2/non oil-absorbing Fe. The utilization efficiency of .OH is improved by H2O2/oil-absorbing Fe. Further analysis found that the oil-absorbing Fe could bind with hydrophobic humic-like acid and fulvic-like acid, increasing the hydrophobicity of oil-absorbing Fe. Thus, macro-crude oil could be efficiently oxidized in soil by oil-absorbing Fe catalyzing H2O2.