Microalgae are a promising feedstock for alternative fuel for compression ignition engines due to their positive contribution to reducing greenhouse gas emissions. Microalgae are gaining significant interest as they can produce more oil than any oilseed plants. Unlike some other plant oils, the use of microalgae as an alternative to fossil fuels, can overcome food versus fuel conflict. In the current investigation, five different chemical components of microalgae hydrothermal liquefaction (HTL) biocrude paraffin, xylene, cyclo-pentanone, dioctylphthalate and butanol were mixed in equal volumes. Commercial diesel fuel was used as a reference fuel. The first blend consisted of 5 vol% of each of the 5 components was mixed with 75 vol% diesel. The blend is called as B1. The second blend consisted of 10 vol% of each of the 5 components was mixed with 50 vol% of diesel. This blend is called B2. The neat diesel (100% pure diesel) is called as 100D. The engine used in this study was a six-cylinder high-pressure common rail direct injection diesel engine fitted with a turbocharger. This study deals with engine performance, combustion and exhaust emission characteristics comparing diesel, B1 and B2 blends. The experimental results indicated a general reduction in both particle mass (PM) and total particle number (PN) emissions with both blends compared to those of diesel. Increase in nitrogen oxides (NOx) emissions at all four engine loads were found with both B1 and B2 blends. It was realised that the drop or rise of emissions was mainly a function of fuel-bound oxygen.