The volumetric, acoustic and viscometric properties are used for investigating the interactions of L-serine and L-arginine in aqueous solutions of a vitamin, namely, thiamine hydrochloride, over a temperature range of (293.15-313.15) K and at atmospheric pressure. The limiting apparent molar volume, V-phi(0), and empirical parameter, S-v, is evaluated from apparent molar volume, V-phi. Similarly, limiting apparent molar isentropic compression, K-phi,s,(0) and slope, S-k, is evaluated from apparent molar isentropic compression, K-phi,(s). The corresponding transfer properties (Delta V-tr(phi)0 and Delta K-tr(phi,s)0) from water to aqueous vitamin solutions are also evaluated. The viscosity data has also been used to analyze relative viscosity, eta(r), viscosity B-coefficients and temperature derivative of B-coefficients, dB/dT. The various calculated parameters assist to procure valuable information regarding the presence of different kinds of intermolecular interactions in the ternary (L-serine/L-arginine + water + thiamine-HCl) solutions. (C) 2019 Elsevier Ltd.