Quick, inexpensive and accurate methods are needed for glucose determination in many biochemical and medical labs. The purpose of this study is to develop and test a novel inexpensive electrode based on nickel/gamma-aminopropyltriethoxysilane nanoparticles for measuring glucose concentrations. First, the Ni nano-particles (Ni NPs) were synthesized by a bottom-up approach. Then, the Ni NPs and gamma-Aminopropyltriethoxysilane (KI-1550) were mixed at 60 degrees C under a nitrogen atmosphere to produce Ni/KH550 nanocomposite. Afterwards, the synthesized Ni/KH550 nanocomposites were characterized using different methods such as Fourier transform infrared (FT-IR), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD). Next, the surface of a glassy carbon electrode (GCE) was polished and washed with alumina powder and deionized water, respectively; and ultrasonicated in the H2O/ethanol solution. Then, the nanocomposite suspension was pipetted dropwise on the GCE surface and dried to GCE-Ni/KH550 production. The newly prepared electrode was characterized and applied to glucose detection utilizing electrochemical methods. The cyclic voltammetry measurements showed that the oxidation peak of glucose appeared at a potential of about 0.53 V (vs. Ag/AgCl) on the surface of the modified GCE. Differential pulse voltammetry exhibited two wide linear dynamic ranges of 0.5-20 and 100-500 mu M glucose with a detection limit of 0.043 mu M. Finally, measurements of glucose in a real sample using our designed sensor indicated that GCE-Ni/KH550 is as good as those obtained with a high efficiency, commercial apparatus.