One of the best ways to increase the fiber content in the diet is supplementing wheat bran in bread. Wheat bran is known to affect the cellular structure of the dough, resulting in lower final bread volume. The present study was focused on analyzing the effect of wheat bran in throughout the bread making process; the results were compared with the recipe without bran. Wheat bran at different level viz., 0%, 8%, 16% and 24% w/w on flour basis was added into the wheat flour for the preparation of sandwich bread. Results showed that wheat bran significantly (p<0.05) affected the dough kneading parameters. Dough properties like stickiness and extensibility decreased with respect to increase in bran quantity. Unexpectedly, wheat bran enhanced the aeration of dough during mixing and the expansion rate of dough during fermentation. However, wheat bran decreased the specific volume of bread upto 10.81%, which was confirmed using 2D image analysis method where it was observed as the wheat bran alters the pore size distribution in bread crumb.