Extraction efficiency of bioactive compounds from yacon leaves using supercritical CO2 + ethanol (scCO(2) + EtOH) fluid extraction and a conventional method were investigated. scCO(2) + EtOH runs were performed under the following conditions: 30-70 degrees C, 15-25 MPa and varying the ethanol (co-solvent) to solid (raw material) mass ratio (MR). All extractions were evaluated in terms of overall extraction yield (X-0), fatty acid composition, total phenolic content (TPC) and antioxidant activity (AA). Results indicated that scCO(2) + EtOH presented the highest extraction yield 5.22 wt% at 70 degrees C/25 MPa/MR 3:1. High values of TPC and AA were found in all extracts. Major unsaturated fatty acids found in yacon leaves extracts were gamma-linolenic (omega-6), eicosapentaenoic (omega-3) and linoleic (omega-6) acids. The highest omega-6/omega-3 fatty acids ratios were found in the extracts obtained using scCO(2) + EtOH.