This report details the use of a Si Metal Oxide Semiconductor Field Effect Transistor (MOSFET) with the gate connected to a disposable antibody-functionalized electrode for the detection of beta-2-transferrin (beta 2T) as a marker of cerebral spinal fluid (CSF). Through the design of a Si based PCB the need for GaN High Electron Mobility Transistors has been mitigated and brings the cost of production for commercial application closer to realization. Through the use of a double pulse method to the test electrode and the drain of the Si MOSFET, ionicity of the test solution is no longer a concern as a double layer is produced and perturbed on the test electrode giving rise to current changes related to the concentration of the biomarker. Additionally, the PCB provides connections for an oscilloscope readout or an onboard display entirely removing the need for any expensive semiconductor parameter analyzers. Clinically, the cutoff for beta 2T as indicator of CSF leakage has traditionally been limited to similar to 2 mu g/mL and takes several hours; with this newly designed board, CSF was detected at levels from 0.1 ng/mL to 100 mu g/mL within 5 minutes, greatly improving the detection limits and feedback time for medical professionals. (C) 2019 The Electrochemical Society.