Organic semiconductors are among the most promising next generation materials for biosensors as the greener and cheaper alternative to transition metal-based electrodes. This work focuses on the production and analysis of organic p-type semiconductor-based glucose biosensors. The compressed graphite electrodes have electrochemically been modified with 9-ethyl-9H-carbazole (CzEt), 9-phenyl-9H-carbazole (CzPh), N, N, N-triphenylamine (TPA) and with cross-linked glucose oxidase (GOx). Response of the created biosensors to the changing concentration of glucose has been assessed by cyclic voltammetry. The electrochemical impedance of these organic semiconductor layers was investigated by using an electrochemical impedance spectroscopy method. Herein, we aim at drawing a picture of the electro-polymerized derivatives and the applicability of p-type carbazole and triphenylamine-based organic semiconductors for amperometric biosensors. (C) 2019 The Electrochemical Society.