A yeast (Saccharomyces cerevisiae EBY100) surface displayed rice a-galactosidase II (YSD rice alpha-Gal II) was constructed. The optimum temperature and pH of YSD rice alpha-Gal II were 45 degrees C and 4.0-5.5, respectively. Ascorbic acid significantly increased the expression level of YSD rice alpha-Gal H by 5.7-fold, compared to control, indicating its importance in the production and application of YSD rice alpha-Gal II. Meanwhile, protective agents including Fe3+, EDTA, urea, and L-arginine also enhanced YSD rice alpha-Gal II activity by a certain extent (15-78%). Furthermore, YSD rice alpha-Gal H exhibited higher thermal, pH, and storage stability, and remarkable hydrolytic activity toward defatted soybean meal and guar gum. Hence, YSD rice alpha-Gal II has great potential for degradation of the non-digestible and flatulence-causing oligo- or poly-saccharides and could be a commendable candidate for extensive application in food and feed industries.