To overcome the deficiency in which 2-Deoxy-D-ribose-5-phosphate aldolase (DERA) exhibits low levels of catalytic efficiency in aldol reactions and poor tolerance for high concentrations of aldehyde, two novel deoC genes encoding DERA(Pyc) and DERA(Met) were identified in Pyrobaculum calidifontis and Meiothermus ruber that had heterologous expression in E. colt BL21. The specific activities toward 2-Deoxy-D-ribose-5-phosphate (DR5P) of DERA(Pye) and DERA(Met) were 13.6 +/- 0.1 and 85.2 +/- 0.7 U/mg, respectively. Almost 56.2% (DERA(Pyc)) and 21.7% (DERA(Met)) of their activity remained after being incubated for 6 h in 300 mM acetaldehyde at 25 degrees C. In addition, the DR5P concentration in the reaction catalyzed by DERA(Pyc) and DERA(Met) was almost 0.31 and 2.63 times the amount catalyzed by DERA(Eco) after reacting for 6 h, respectively. These results suggest that these two novel DERAs with a high tolerance for a high concentration of aldehyde have the potential to be widely used in industrial settings.