In this study, we propose the application of a Continuous Two-Phase Partitioning Bioreactor (C-TPPB) operated with the tubing effluent recycle to enhance the biodegradation of toxic substrates in industrial wastewaters under severe loading conditions. Stepwise increasing influent concentrations (from 200 to 900 mg L-1) of 2,4-dichlorophenol (2,4-DCP) were fed to a C-TPPB operated with Hytrel G3548 tubing to simulate phenolic wastewater. Practically complete 2,4-DCP removal has been achieved during the entire experimental period, but the increased load reduced biodegradation efficiency. At influent concentration of 700 mg L-1, the first effluent recycle (recycle /influent flow rate ratio = 0.3) was applied: biodegradation efficiency doubled from 40 to 80% and was maintained until influent concentrations of 800 mg L-1. Higher influent concentrations caused a decrease in 2,4-DCP biodegradation, so the effluent recycle ratio was increased to 0.5 at 900 mg L-1 and, also in this case, the bioreactor showed a fast recovery (similar to 24 h) of the biodegradation efficiency at 80%. Mass transfer data analysis showed that the effluent recycle resulted in an increase of the mass transfer coefficient. This positive effect, joined with the reduction of the influent concentrations, demonstrated the feasibility of recycle application in enhancing the C-TPPB performance. (C) 2019 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.