Factors affecting flocculating properties of two oleaginous yeasts, Candida tropicalis X37 and Yarrowia lipolytica TISTR 5151 were evaluated in order to simplify harvesting process and increase process productivity. Among these two yeasts, Y. lipolytica TISTR 5151 showed high flocculation efficiency (>95%) at acidic pH while C. tropicalis X37 gradually flocculated independently to pH. Both yeasts and their mixed culture grew well and accumulated high lipid content (>30%) in palm oil mill effluent added with crude glycerol at 1-2%. Interestingly, the mixed culture enhanced lipid productivity and COD removal possibly due to stabilization of pH and efficient organic carbon consumption. Y. lipolytica TISTR 5151 with appropriate inoculum size could also be used as bioflocculent for other yeasts. The fatty acid composition of yeast lipids and their prospect fuel properties indicate their potential use as biodiesel feedstocks. (C) 2018 Published by Elsevier Ltd.