화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.77, 243-252, September, 2019
DOI10.1016/j.jiec.2019.04.045  Export Citation
Synthesis of polythiophene nanoparticles by surfactant-free chemical oxidative polymerization method: Characterization, in vitro biomineralization, and cytotoxicity evaluation
Deval Prasad Bhattarai1, 2, Ganesh Prasad Awasthi1, Bikendra Maharjan1, Joshua Lee1, Beom-Su Kim3, †, Chan Hee Park1, 4, †, and Cheol Sang Kim1, 4, †
  • 1Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea
  • 2Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • 3Carbon Nano Convergence Technology Center for Next Generation Engineers (CNN), Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea
  • 4Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea
E-mail:, ,
Herein, we report the synthesis of polythiophene nanoparticles (PTh-NPs) by surfactant-free chemical oxidative polymerization of thiophene at 37 °C using ammonium persulphate as oxidant. PTh-NPs synthesized without surfactant were compared to those with surfactant in terms of surface morphology, crystallinity, cytotoxicity and some other aspects. Thermogravimetric analysis showed a good thermal stability of as-synthesized PTh-NPs. In vitro biomineralization revealed the nucleation of calcium and phosphate onto the NPs. Cytotoxicity of PTh-NPs was evaluated by measuring cell viability of preosteoblast MC3T3-E1 and PC12 cell lines. PTh-NPs synthesized without using surfactant exhibited better cell viability compared to those with surfactant.
Keywords:Polythiophene nanoparticle;Chemical polymerization;Biomineralization;MC3T3-E1 cell;PC12 cell;Cytotoxicity
  1. Rincon C, Meredith JC, Macromol. Biosci., 10, 258 (2010)
  2. Tuncel D, Demir HV, Nanoscale, 2, 484 (2010)
  3. Pecher J, Mecking S, Chem. Rev., 110(10), 6260 (2010)
  4. Lee SJ, Lee JM, Cho HZ, Koh WG, Cheong IW, Kim JH, Macromolecules, 43(5), 2484 (2010)
  5. Nalwa HS, Handbook of Nanostructured Materials and Nanotechnology, Five-Volume Set, Academic Press, 1999.
  6. Uleanya K, Eboatu A, Pac. J. Sci. Technol., 15, 200 (2014)
  7. Bhattarai DP, Tiwari AP, Maharjan B, Tumurbaatar B, Park CH, Kim CS, J. Colloid Interface Sci., 534, 447 (2019)
  8. Smela E, Adv. Mater., 15(6), 481 (2003)
  9. Nezakati T, Seifalian A, Tan A, Seifalian AM, Chem. Rev., 118(14), 6766 (2018)
  10. Gajendiran M, Choi JH, Kim SJ, Kim KS, Shin HS, Koo HJ, Kim KB, J. Ind. Eng. Chem., 51, 12 (2017)
  11. Peart PA, Repka LM, Tovar JD, Eur. J. Org. Chem., 2008, 2193 (2008)
  12. Jaymand M, Sarvari R, Abbaszadeh P, Massoumi B, Eskandani M, Beygi-Khosrowshahi Y, J. Biomed. Mater. Res. A, 104, 2673 (2016)
  13. Hatamzadeh M, Jaymand M, RSC Adv., 4, 16792 (2014)
  14. Bora C, Pegu R, Saikia BJ, Dolui SK, Polym. Int., 63, 2061 (2014)
  15. Guo H, Zhu H, Lin H, Zhang J, Yu L, J. Disper. Sci. Technol., 29, 706 (2008)
  16. Karim MR, Lim KT, Lee CJ, Lee MS, Synth. Met., 157, 1008 (2007)
  17. Ambade RB, Ambade SB, Shrestha NK, Salunkhe RR, Lee W, Bagde S, Kim JH, Stadler FJ, Yamauchi Y, Lee SH, J. Mater. Chem. A, 5, 172 (2017)
  18. Wang X, Shi G, Liang Y, Electrochem. Commun., 1, 536 (1999)
  19. Aeiyach S, Bazzaoui EA, Lacaze PC, J. Electroanal. Chem., 434(1-2), 153 (1997)
  20. Yagci Y, Yilmaz F, Kiralp S, Toppare L, Macromol. Chem. Phys., 206, 1178 (2005)
  21. Ryu HW, Kim YS, Kim JH, Cheong IW, Polymer, 55(3), 806 (2014)
  22. Zong XP, Miao XA, Hua SX, An L, Gao XA, Jiang WS, Qu D, Zhou ZJ, Liu XY, Sun ZC, Appl. Catal. B: Environ., 211, 98 (2017)
  23. Oh WK, Kim S, Choi M, Kim C, Jeong YS, Cho BR, Hahn JS, Jang J, ACS Nano, 4, 5301 (2010)
  24. Bendrea AD, Cianga L, Cianga I, J. Biomater. Appl., 26, 3 (2011)
  25. Huang ZB, Yin GF, Liao XM, Gu JW, Front. Mater. Sci., 8, 39 (2014)
  26. Nel A, Xia T, Madler L, Li N, Science, 311, 622 (2006)
  27. Cianga L, Bendrea AD, Fifere N, Nita LE, Doroftei F, Ag D, Seleci M, Timur S, Cianga I, RSC Adv., 4, 56385 (2014)
  28. Adhikari MD, Goswami S, Panda BR, Chattopadhyay A, Ramesh A, Adv. Healthc. Mater., 2, 599 (2013)
  29. Jeong YS, Oh WK, Kim S, Jang J, Biomaterials, 32, 7217 (2011)
  30. Karim MR, Lee CJ, Lee MS, J. Polym. Sci. A: Polym. Chem., 44(18), 5283 (2006)
  31. Baig U, Gondal MA, Alam MF, Alam M, Wani WA, Younus H, Int. J. Polym. Mater. Polym. Biomater., 66, 243 (2017)
  32. AshaRani PV, Low Kah Mun G, Hande MP, Valiyaveettil S, ACS Nano, 3, 279 (2009)
  33. Bhattarai DP, Shrestha S, Shrestha BK, Park CH, Kim CS, Chem. Eng. J. (2018).
  34. Wang Z, Wang Y, Xu D, Kong ESW, Zhang Y, Synth. Met., 160, 921 (2010)
  35. Kadac K, Nowaczyk J, J. Appl. Polym. Sci., 133 (2016)
  36. Zabihi O, Khodabandeh A, Mostafavi SM, Polym. Degrad. Stabil., 97, 3 (2012)
  37. Iqbal S, Shah J, Kotnala RK, Ahmad S, J. Alloy. Compd., 779, 487 (2019)
  38. Wu KZ, Zhao JJ, Wu RT, Ruan B, Liu HT, Wu MX, J. Electroanal. Chem., 823, 527 (2018)
  39. Gnanakan SRP, Rajasekhar M, Subramania A, Int. J. Electrochem. Sci., 4, 1289 (2009)
  40. Lee SJ, Lee JM, Cheong IW, Lee H, Kim JH, J. Polym. Sci. A: Polym. Chem., 46(6), 2097 (2008)
  41. Liu R, Liu Z, Chin. Sci. Bull., 54, 2028 (2009)
  42. Han MG, Foulger SH, Adv. Mater., 16(3), 231 (2004)
  43. Udum YA, Pekmez K, Yıldız A, Synth. Met., 142, 7 (2004)
  44. Mohammad F, J. Phys. D-Appl. Phys., 31, 951 (1998)
  45. Jacobs C, Kayser O, Muller RH, Int. J. Pharmaceut., 196, 161 (2000)
  46. Clogston JD, Patri AK, Zeta Potential Measurement, Humana Press, Totowa, NJ, p.63 2011.
  47. Jeon SS, Yang SJ, Lee KJ, Im SS, Polymer, 51(18), 4069 (2010)
  48. Senatov F, Niaza K, Zadorozhnyy MY, Maksimkin A, Kaloshkin S, Estrin Y, J. Mech. Behav. Biomed. Mater., 57, 139 (2016)
  49. Kelkar DS, Chourasia AB, Indian J. Phys., 86, 101 (2012)
  50. Yao Y, Gao J, Bao F, Jiang S, Zhang X, Ma R, RSC Adv., 5, 42754 (2015)
  51. Yang R, Wang S, Zhao K, Li Y, Li C, Xia Y, Liu Y, Polym. Sci. Ser. B, 59, 16 (2017)
  52. Song Y, Zhang S, Li J, Zhao C, Zhang X, Acta Biomater., 6, 1736 (2010)
  53. Garay-Jimenez JC, Young A, Gergeres D, Greenhalgh K, Turos E, Nanomed. Nanotechnol. Biol. Med., 4, 98 (2008)
  54. Sayed AEDH, Authman MMN, Ecotoxicol. Environ. Saf., 163, 136 (2018)
  55. Ahmed KBR, Nagy AM, Brown RP, Zhang Q, Malghan SG, Goering PL, Toxicol. Vitro, 38, 179 (2017)
  56. Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK, Beilstein J. Nanotechnol., 9, 1050 (2018)
  57. Li DF, Wang HJ, Fu JX, Wang W, Jia XS, Wang JY, J. Phys. Chem. B, 112(51), 16290 (2008)
  58. Vaitkuviene A, Kaseta V, Voronovic J, Ramanauskaite G, Biziuleviciene G, Ramanaviciene A, Ramanavicius A, J. Hazard. Mater., 250-251 167-174 (2013).
  59. Chen H, Yuan L, Song W, Wu Z, Li D, Prog. Polym. Sci, 33, 1059 (2008)
  60. Laurent S, Burtea C, Thirifays C, Hafeli UO, Mahmoudi M, PLoS One, 7, e29997 (2012)
  61. Bhattarai D, Aguilar L, Park C, Kim C, Membranes, 8, 62 (2018)
  62. Marrella A, Lee TY, Lee DH, Karuthedom S, Syla D, Chawla A, Khademhosseini A, Jang HL, Mater. Today, 21, 362 (2018)