Recently, nanoparticle-based immobilization of biocatalytic systems is getting interested in bioremediation efficiency. Therefore, hollow nanosphere particles of TiO2 - Cu (< 50 nm) were used to immobilize P450 BM3 enzyme produced by engineered E. coli to create an effective novel material that can be significantly used for organic pollutants degradation under solar radiation conditions. Scanning Electron Microscope (SEM) and the evaluation of enzyme activity before and after immobilization step were used to confirm the successful immobilization process of enzyme on the surface of hollow nanosphere TiO2 - Cu composite. The enzyme P450 BM3 was strongly immobilized on hollow nanosphere composite and its activity was doubled than that of the free enzyme produced by batch fermentation of engineered E. coli. Furthermore, the catalytic potential of P450 BM3 - hollow nanocomposite biocatalyst was examined for in vitro degradation of isopropanol as a model of organic gases pollutants under visible radiation. It was found that the degradation of isopropanol was high (95%) with enzyme- hollow nanosphere composite biocatalyst (5 mg) at an initial condition of pH ((similar to)7.0), ambient temperature, and isopropanol concentration (20 mg/L). Control experiment indicated that P450 BM3 enzyme- hollow nanosphere composite biocatalyst was better than both the hollow nanosphere composite without enzyme and free enzyme. Therefore, the hollow nanocomposite TiO2 - Cu seemed to enhance stability and activity of immobilized P450 BM3 enzyme over the free enzyme. Moreover, a combination of nanomaterial and enzyme is required for achieving biocompatibility and inert condition without denaturing the enzyme. Overall, TiO2 -Cu hollow nanosphere composite is potential for large-scale P450 BM3 immobilization with improved properties and reuse. The results showed that the performance of nanobiocatalyst was successfully introduced via combined biocatalytic property of the enzyme and photocatalytic property of the hollow nano sphere composite. These were carried out for the first time in this paper. Therefore, this system is a potentially valid approach for air pollution remediation with a bright future in industrial applications as a new photonanobiocatalyst.