화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.30, No.4, 408-414, August, 2019
DOI10.14478/ace.2019.1032  Export Citation
BBD-RSM을 이용한 민들레로부터 항산화성분의 추출공정 최적화
Optimization of Antioxidant Extraction from Dandelion (Taraxacum officinale) Leaves Using BBD-RSM
한경호, 장현식1, 홍인권1, †
  • 단국대학교 전자전기공학부
  • 1단국대학교 화학공학과
Kyongho Han, Hyun Sik Jang1, and In Kwon Hong1, †
  • School of Electronics and Electrical Engineering, Dankook University, Gyeonggi 16890, Korea
  • 1Department of Chemical Engineering, Dankook University, Gyeonggi 16890, Korea
E-mail:
초록
본 연구에서는 전통적인 열수추출법과 초음파 추출법을 이용하여 민들레에서 항산화성분을 추출하였다. 추출 후 추출 수율과 항산화성분인 플라보노이드 함량을 최적화하기 위해 반응표면분석법 중 Box-Behnken 설계모델을 이용하여 추출하였다. 열수추출법의 경우 추출온도, 주정/초순수 부피비, 추출시간을 변수로 설정하였으며, 초음파 추출법의 경우 초음파 조사세기, 주정/초순수 부피비, 초음파 조사시간을 변수로 설정하였다. 열수추출법의 최적 추출조건은 추출 온도(45.76 ℃), 주정/초순수 부피비(41.92 vol.%), 추출시간(1.75 h)이었다. 또한 초음파 추출법의 최적조건은 초음파 조사세기(512.63 W), 주정/초순수 부피비(56.97 vol.%), 추출시간(20.79 min)이었다. 이 조건에서 예상되는 반응치는 수율(22.09 wt.%), 플라보노이드 함량(28.98 mg QE/mL dw)으로 산출되었다. 또한 실제실험 결과 오차는 3% 이하로 나타나 최적화의 타당성을 입증하였다.
In this study, an antioxidant was extracted from dandelion leaves using traditional hot water and ultrasonic extraction methods. In order to optimize the extraction yield and total flavonoid, an antioxidant, Box-Behnken design (BBD) model among response surface analysis methods was used. In the case of hot water extraction, the extraction temperature and time as well as the ratio of alcohol/ultrapure water were set as variables, and for the ultrasonic extraction, the ultrasonic survey century and irradiation time and the ratio of alcohol/ultrapure water were variables. Optimum extraction conditions in the hot water extraction method were the extraction temperature and time of 45.76 ℃ and 1.75 h and the ratio of alcohol/ultrapure water of 41.92 vol.%. While for the ultrasonic extraction method the survey century of 512.63 W, the ratio of alcohol/ultrapure water of 56.97 vol.% and the extraction time of 20.79 min were optimum conditions. Expected reaction yield and flavonoid content values under the optimized condition were calculated as 22.09 wt.% and 28.98 mg QE/mL dw, respectively. In addition, the error value of less than 3% was obtained validating our optimization process.
Keywords:Total flavonoids;Antioxidant;Dandelion leaves;Box-Behnken design model
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