Poly(n-butyl methacrylate) (PBMA) or poly(n-butyl acrylate) (PBA)-grafted brush coatings attached to glass were successfully prepared using atom-transfer radical polymerization "from the surface". The thicknesses and composition of the PBMA and PBA coatings were examined using ellipsometry and time-of-flight secondary ion mass spectrometry (ToF-SIMS), respectively. For PBMA, the glass-transition temperature constitutes a range close to the physiological limit, which is in contrast to PBA, where the glass-transition temperature is around -55 degrees C. Atomic force microscopy studies at different temperatures suggest a strong morphological transformation for PBMA coatings, in contrast to PBA, where such essential changes in the surface morphology are absent. Besides, for PBMA coatings, protein adsorption depicts a strong temperature dependence. The combination of bovine serum albumin and anti-IgG structure analysis with the principal component analysis of ToF-SIMS spectra revealed a different orientation of proteins adsorbed to PBMA coatings at different temperatures. In addition, the biological activity of anti-IgG molecules adsorbed at different temperatures was evaluated through tracing the specific binding with goat IgG.