Copper oxide (CuO) nanoparticles (NPs) were prepared through a biological procedure where the Rosa canina fruit extract was used as a capper and reductant agent. For the three-component reaction of amines, aldehydes and alkynes (A(3) coupling), these CuO NPs were used as an effective heterogeneous nanocatalyst. Various propargylamines were obtained in a good yield. Additionally, the reuse and separation of CuO NPs was extremely effective, economical and simple. [GRAPHICS] .