The problem of mixed convection heat transfer inside a horizontal vented enclosure through the lower and upper parts, respectively, of its left and right vertical walls is studied numerically using Al2O3-water nanofluid as working fluid. The bottom wall is subjected to a linearly varying (increasing or decreasing) heating temperature profiles, while the other boundaries are considered thermally insulated. The fresh fluid is admitted from the bottom part of the left vertical wall by injection or by the suction imposed on the opening of the right vertical wall. Based on numerical predictions, the conjugate effect of the Reynolds number and the nanoparticle concentration on fluid flow and heat transfer characteristics is studied. The obtained results demonstrate clearly the positive role of the nanoparticles addition on the improvement of the heat transfer rate and the mean temperature within the cavity. In addition, the flow structure and the temperature distribution inside the cavity are seen to be very sensitive to the variations of the Reynolds number, the imposed external flow mode, and the heating type. Results presented show that, in general, the decreasing heating mode is more favorable to the heat transfer in comparison with the case of the increasing heating mode. The cooling efficiency is found to be more pronounced by the injection/suction mode by applying the increasing/decreasing heating type.