In this work, advanced x-ray radiographic techniques available at the Canadian Light Source (CLS) were utilized to study water droplet dynamics in a serpentine flow channel mimicking a proton exchange membrane fuel cell (PEMFC). High spatial and temporal resolution coupled with high energy photons of an x-ray beam provided high-resolution images of water droplets. This technique solved the problem caused by the opaqueness of fuel cell materials including the gas diffusion layer by providing a unique way to study water droplet dynamics at different operating conditions. From the captured images, droplet emergence and formation on porous gas diffusion layers (GDLs) were analyzed. Three commercially available GDLs (Sigracet AA, Sigracet BA, and Sigracet BC) were used and droplet detachment height was found to decrease in the following order AA < BA < BC under the same flow condition. Increasing the superficial gas velocity was found to decrease the droplet detachment height for all GDLs tested. Average droplet cycle for various operating conditions was obtained. It was found that humidified air did not show a difference in droplet dimensions at detachment compared to dry air used at the inlet gas. However, it did show an impact on droplet cycle time, which might be due to condensation. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.