Heteroatoms-doped carbon-based materials (with non-precious metals or no metals) with porous structure have already shown high catalytic activities for oxygen reduction reaction (ORR), especially in microbial fuel cells (MFCs). Here, we use pectin extracted from pomelo peels as carbon source to prepare metal-free and sulphur/nitrogen co-doped partially-graphitized carbon (HP-SN-PGCs) by using silica nanospheres as sacrificial templates. Single-chamber MFC (SC-MFC) with HP-SN-PGC-0.5 (0.5 g of silica) cathode has the shortest start-up time (45 h) and lowest charge transfer resistance (19.3 Omega). The maximum power density of HP-SN-PGC-0.5 (1161.34 mW m(-2)) cathode is higher than that of Pt/C (1116.90 mW m(-2)) at the initial cycle. After 75 d operation, power density of HP-SN-PGC-0.5 cathode only declines 4.6%, which is more stable than that of Pt/C (37.69%). HP-SN-PGC-0.5 has a highly porous structure (869.25 m(2) g(-1)) by removal of templates and Fe species (as the graphitization catalyst) to facilitate exposure of active sites and diffusion of ORR-related intermediates (OH- and HO2-, etc) to accessible active sites. N and S species provide highly active sites to enhance OH- generation to conduct the 4e(-) ORR process. Thus, this study presents a viable ORR catalyst with high activity and long-term stability for bioelectricity generation from organic wastewater in SC-MFCs. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.