This study evaluated the effect of air-abrasion protocols on the topography, surface wettability and adhesion of resin cement to zirconia. Ceramic specimens (N=49; n=7) (15mm x 2mm) were randomly allocated to seven groups to be treated with: (1) Air-abrasion with 45 mu m Al2O3 (A45), (2) 80 mu m Al2O3 (A80), (3) 30 mu m Al2O3 coated with SiO2 (CoJet) (C30), (4) 30 mu m Al2O3 coated with SiO2 (Rocatec Soft) (R30), (5) 110 mu m Al2O3 coated with SiO2 (Rocatec Plus) (R110); (6) R110R30 (Rocatec) (R110R30) and (7) control, no conditioning (NC). Air-abrasion was performed using a chairside air-abrasion device (2.5bar, 10mm, 90s). Contact angle measurements were performed using goniometry (n=5). MDP-based dual resin cement (Panavia F2.0) was bonded on four locations after air-abrasion protocols (n=20 per group). Half of the specimens were tested after 24h and the other half after thermal cycling (x3000, 5-55 degrees C). Data were analyzed using 1-, 2-way ANOVA and Tukey's test (alpha = 0.05). Significantly lower contact angle values were observed for groups C30 (62.6 +/- 0.91), R30 (61.91 +/- 1.05) and R110R30 (61.54 +/- 1.02) compared to those of other groups (65.5 +/- 0.9-110.61 +/- 0.9) (p<0.05). In dry conditions, surface conditioning methods tested did not show significant effect on bond strength (MPa) (10.57 +/- 1.42-16.86 +/- 2.54) (p=0.238). After thermocycling, bond strength results decreased significantly (p<0.05) (12.6-51.2%). R110 (7.18 +/- 1.34) and A80 (4.92 +/- 1.53) showed significantly higher bond strength compared to other groups (2.13 +/- 0.73-4.16 +/- 1.34) (p<0.05). The best wettability and adhesion results with MDP-based resin cement to zirconia was achieved with A80 and R110 air-abrasion.