BACKGROUND Enzymatic catalysis in a microchannel under continuous flow has attracted increasing scientific interest. It allows for surfaces with higher specificities for heat and mass transfer and makes it easier to control reaction parameters. However, its applications are limited by a lesser amount of loading and cycle of use of the immobilized enzyme. A series of groove-typed channel microreactors with surface geometry patterns were first established for effective immobilization of naringinase, which was used to produce isoquercitrin from rutin via hydrolysis. RESULTS Of the tested groove-typed channel microreactors with surface geometry patterns, the enzyme adsorbing capacities of all were increased compared to that with smooth surface. The enzyme adsorbing capacity increased 1.32-fold in the grooved microreactor with triangular pattern. Excellent yields of isoquercitrin production were produced in grooved channel immobilized enzyme microreactors with triangular surfaces. A yield of 91.72 +/- 0.65% was obtained in 10 min, the microreactor could be reused 12 times with a residual activity over 56%. Efficient transfer of heat and mass were attained in the grooved microreactors with surface geometry patterns. The Poiseuille number and Nusselt number were increased by 20.61% and 29.63%, respectively, using fluid mechanics analysis in the grooved microreactor with triangular pattern. CONCLUSION The grooved microreactor with geometry pattern surface provided an efficient method for the high production of isoquercitrin, which is promising for enzyme immobilization and catalysis. (c) 2019 Society of Chemical Industry