Carvacrol (CAR) is a terpenoid with strong antioxidant, antimicrobial and antitumor properties. Research has shown that carvacrol is effective in the treatment of gastric cancer. Despite the low side effects of carvacrol compared to the chemical drugs, its solubility is low in water. It is one of the challenging limitation of this compound for the disease treatment. To overcome this problem, albumin has been used for its transport in blood. Albumin has a set of quite diverse functions such as binding and transport capacities of drugs and antioxidants. Therefore, recently, the interaction between many herbal medicines with albumin has been considered. In this study, interaction between bovine serum albumin (BSA) and CAR was investigated with the aim of developing a new method for the determination of BSA. For this purpose, the interaction between BSA and CAR was investigated using electrochemical methods such as cyclic voltammetry (CV), differential pulse voltammetry (DPV), square wave voltammetry (SWV) and spectroscopic (UV-Vis) methods. The chemometric tools including multivariate curve resolution-alternating least square (MCR-ALS) and parallel factor analysis (PARAFAC) have been used to achieve more information about this interaction. Also, molecular docking studies were carried out to clarify the binding poses of carvacrol at the binding pockets of albumin. The obtained results reveal that there Is a strong interaction between carvacrol and BSA. Finally, DPV technique was further applied for the determination of BSA at the RGO/GC modified electrode. The obtained results from differences of DPV responses of CAR in the absence and presence of BSA (Delta I) revealed a linear range from 1.0 to 45.0 mu M and the detection limit of 0.34 mu M for BSA.