Short-time aerobic digestion (STAD) was proved to promote the reduction of waste activated sludge (WAS). This study systematically disclosed the influential characteristics and mechanisms of linear alkylbenzene sulfonates (LAS) dosage on the reduction of WAS in STAD system. Flow cytometer (FC) combined with SYTOX Green (SG) dye was used to differentiate extracellular polymeric substances (EPS) release and cell lysis of WAS during STAD process. LAS lower than 0.10 g/g total suspended solids (TSS) brought about EPS solubilization and the decrease of sludge floc size, and the accumulated soluble microbial products (SMP) could be biodegraded by heterotrophs. Moreover, the activity of microorganisms (denoted as specific oxygen uptake rate (SOUR)) and proportion of bacteria functional for LAS and SMP biodegradation dramatically increased, leading to a high LAS biodegradation rate (k(LAS)) and increased WAS biodegradation rate (k(COD), (WAS)). Even more LAS (> 0.10 g/g TSS) caused cell lysis, leading to the decreased k(TCOD) and k(LAS), and therefore inhibit the reduction of WAS. High WAS reduction and LAS biodegradation rate were achieved at the LAS dosage of 0.10 g/g TSS in STAD system. This study lays the foundation for improving WAS reduction by optimizing surfactant dose in STAD system.