Acrylonitrile butadiene rubber (NBR)-based nanocomposites reinforced with organo-clay, calcium carbonate, and silica as nanofillers with different contents ranging from 1 to 10phr (part per hundred parts of rubber) were prepared and characterized. Surface modification was done on the clay nanoparticles to intensify the formation of chemical bond between nanofiller and NBR matrix. A novel procedure and formula were implemented to fabricate considered composites and nanocomposites. Variety of tests and analyzes were done on all nanofillers and fabricated nanocomposites and accordingly, curing conditions, mechanical and chemical properties of the resulting NBR nanocomposites intercalated with nanofillers were further improved and optimized. The nanocomposite containing 10phr of nanoorgano-clay were found to be the best choice among other synthesized nanocomposites in the case of improved curing conditions, mechanical, and chemical properties.