Well-dispersed trimetallic Pd-Ru-Bi alloy nanoparticles (NPs) were successfully embedded in the three-dimensional (3D) rGO/MOF-199 by a facile hydrothermal and in situ impregnation-reduction method. Among the mono-, bi- and tri-metal@rGO/MOF-199 electrocatalysts, the trimetallic PdRuBi@rGO/MOF-199 exhibited the highest catalytic activity and durability towards ethylene glycol oxidation reaction (EGOR) under the alkaline condition, as high as 213.93 mA cm(-2), is about 7.23 times higher than that of commercial Pd/C. The outstanding electrocatalysis oxidation performance of the PdRuBi@rGO/MOF-199 is attributed to the unique 3D structures with trimetallic composition and strong synergistic effects of Pd, Ru and Bi nanoalloys that providing plenty of interfaces and achievable reaction active sites. Furthermore, the introduction of GO in MOF-199 matrix conduces to the superior electron transfer and large surface area, which are beneficial to improve the catalytic ability of the catalyst. The study on the new composites offers a conception and applicable way to develop the advanced Pd-based electrocatalysts for high performance direct ethylene glycol fuel cells.