We report on the transient and steady-state optical responses of the chromophore 2,2’-bithiophene-5,5’-diylbis(phosphonic acid) (BDP) incorporated within a single zirconium-phosphonate layer as a function of chromophore density. While the dilute solution optical response of BDP reveals no anomalous behavior, its characteristics are substantially different when confined within a monolayer. We vary the concentrations of layer constituents to determine the extent of interaction between BDP moieties within a single monolayer. We observe limited initial aggregation of BDP, the extent of which is determined largely by the conditions under which the monolayer is formed. Over time, the fractional contribution of BDP aggregates to the total optical response decreases to a limiting value, implicating surface adsorption site density as the dominant factor in determining the morphology of the organobis(phosphonate) layer. Motional relaxation measurements of BDP within the layer show that the chromophores are immobile on the hundreds-of-picoseconds time scale of our experiments.