In the presence of Cu ions, a packed bed electrochemical reactor (PBER) was employed to deliberately avoid or induce galvanic coupling between pyrite and Cu-activated sphalerite. The effect of galvanic interaction on Cu ions uptake and xanthate adsorption were investigated. Solution chemistry and surface chemistry studies (ethylenediaminetetraacetic acid extraction and time of flight secondary ion mass spectrometry) have observed that when sphalerite and pyrite were galvanically coupled, Cu ions migrated from the pyrite surface to the surface of the sphalerite. Along with the marked decrease in the adsorption of Cu ions on pyrite, xanthate adsorption on the minerals also dramatically dropped. The pseudo-adsorption rate constant for the minerals in the mixed mode is only 0.0583 s(-1), much less than that in the decoupled mode, which is 0.1368 s(-1). This testing program shows that the galvanic coupling of minerals contributes to more copper transfer and Cu ions preferentially adsorbed by sphalerite rather than pyrite. This affects the pyrite surface and causes it to become xanthate unflavoured.