The biosynthesis of Pt-nanoparticles (Pt NPs) supported on bovine bone powder was conducted by an environmentally friendly method that consists on immersing bovine bone powder into a Pt4+ metal ion solution at room temperature, atmospheric pressure and subsequent reduction by Heterotheca inuloides. It is worth pointing out that a calcination process is not required for the synthesis of this catalyst by the method reported herein. The nanocomposite was characterized by transmission electron microscopy (TEM), which revealed uniformly dispersed platinum nanoparticles with quasi-spherical form and average particle size of 7.1 nm. The XPS studies exhibited the presence of 47.62% Pt degrees and 51.84% PtO. The catalyst activity was tested in the selective hydrogenation of 2-butyne-1,4-diol towards 2-butene-1,4-diol. The nanocomposite exhibits a reasonable catalytic performance with nearly 100% conversion of the alkyne and 96% selectivity towards 2-butene-1,4-diol.