Large amounts of fossil fuels are consumed by motor vehicles annually, and hazardous exhaust emissions from the motor vehicles have caused serious problems to environment and human health. Eco-driving can effectively improve the fuel economy and decrease the exhaust emissions, which makes it vital to analyze the fuel consumption and exhaust emissions at given driving cycle, and investigate their sensitivities to eco-driving factors. In this paper, the fuel consumption and exhaust emissions of a Euro-6 compliant light-duty diesel vehicle were tested in Worldwide Harmonized Light Vehicles Test Cycles on a chassis dynamometer; further, the sensitivities of the eco-driving factors that influence the fuel economy and exhaust emissions were analyzed using validated vehicle model. For the vehicle model simulation, the effect of the coolant temperature on fuel consumption and exhaust emission only considered its effect on lubricating oil viscosity. The results showed that vehicle acceleration and velocity dominates the fuel consumption rates in Worldwide Harmonized Light Vehicles Test Cycles, where more than 50% of the exhaust emissions was emitted in the first 300 s; also, fuel economy and exhaust emission factors showed a significant dependency on the road grade, coolant temperature, vehicle velocity and mass. For the driver-controllable factors, high vehicle velocity and low road grade (via route-choice) were recommended to achieve low fuel consumption and exhaust emissions.