Aims To assess the ability of various newly isolated or belonging in official collections yeast strains to convert biodiesel-derived glycerol (Gly) into added-value compounds. Methods and Results Ten newly isolated yeast strains belonging to Debaryomyces sp., Naganishia uzbekistanensis, Rhodotorula sp. and Yarrowia lipolytica, isolated from fishes, metabolized Gly under nitrogen limitation. The aim of the study was to identify potential newly isolated microbial candidates that could produce single-cell oil (SCO), endopolysaccharides and polyols when these micro-organisms were grown on biodiesel-derived Gly. As controls producing SCO and endopolysaccharides were the strains Rhodotorula glutinis NRRL YB-252 and Cryptococcus curvatus NRRL Y-1511. At initial Gly (Gly(0)) approximate to 40 g l(-1), most strains presented remarkable dry cell weight (DCW) production, whereas Y. lipolytica and Debaryomyces sp. produced non-negligible quantities of mannitol and arabitol (Ara). Five strains were further cultivated at increasing Gly(0) concentrations. Rhodotorula glutinis NRRL YB-252 produced 7 center dot 2 g l(-1) of lipid (lipid in DCW value approximate to 38% w/w), whereas Debaryomyces sp. FMCC Y-69 in batch-bioreactor experiment with Gly(0) approximate to 80 g l(-1), produced 30-33 g l(-1) of DCW and similar to 30 g l(-1) of Ara. At shake-flasks with Gly(0) approximate to 125 g l(-1), Ara of similar to 48 g l(-1) (conversion yield of polyol on Gly consumed approximate to 0 center dot 62 g g(-1)) was achieved. Cellular lipids of all yeasts contained in variable concentrations oleic, palmitic, stearic and linoleic acids. Conclusions Newly isolated, food-derived and non-previously studied yeast isolates converted biodiesel-derived Gly into several added-value metabolites. Significance and Impact of the Study Alternative ways of crude Gly valorization through yeast fermentations were provided and added-value compounds were synthesized.